Detection of Bile Salt in Urine

Detection of Bile Salt in Urine :-
Bile is a yellow-green fluid that contains water and organic molecules such as cholesterol, bile acids, and bilirubin. In humans, the two main function of bile are digestion and absorption of fats and eliminating bile salts from the body by secretion into bile. In humans and most vertebrates, bile is produced by the liver.

We can test for the presence of bile salt in urine using Smith’s reagent and Pattern Raffo’s test. On adding urine to the Smith’s reagent, a green ring is formed in the presence of bile salt in urine. Pattern Raffo’s test gives a red colour in the presence of bile salt.

This video explains how to detect the presence of bile salt in urine.
Featured playlist